ANIT-induced disruption of biliary function in rat hepatocyte couplets
نویسندگان
چکیده
منابع مشابه
Hepatocyte couplets.
Hepatocyte couplets, by retaining functional and morphological polarity similar to that of hepatocytes in situ, are a valuable in vitro model to study mechanisms of bile secretion, cholestasis and hepatocellular injury. They have been useful in studies of the hormonal control of bile formation and are suitable for morphological studies. The availability of periportal- and perivenous-enriched co...
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Isolated rat hepatocyte couplets were used to perform the comparative study of two widely used immunosuppressors, cyclosporin A (CsA) and tacrolimus (FK506) on hepatocanalicular function. We assessed canalicular function by counting the percentage of couplets that were able to accumulate the fluorescent cholephile, cholyl-lysyl-fluorescein (CLF), into the canalicular vacuole between the two cel...
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Excitable cells often display rapid coordination of hormone-induced intracellular calcium signals. Calcium elevations that begin in a single epithelial cell also may spread to adjacent cells, but coordination of hormone-induced signals among epithelial cells has not been described. We report the use of confocal microscopy to determine the inter- and intracellular distribution of cytosolic calci...
متن کاملPurification and use of hepatocyte couplets.
Partial digestion of liver with collagenase can provide a cell population composed of single cells (singlets), pairs of cells (couplets) or larger groupings (multiplets) [l]. The individual cells within the couplets and multiplets possess both gap junction and tight junction function. Studies with these polarised primary cells can be aimed, for example, at the biliary pole without a requirement...
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translation as a comunicative process is always said to be associated with various aspects of meaning loss or gain. subtitling as a mode of translating, due to special discoursal and textual conditions imposed upon it, is believed to be an obvious case of this loss or gain. presenting the spoken sound track of a film in writing and synchronizing the perception of this text by the viewers with...
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ژورنال
عنوان ژورنال: Toxicological Sciences
سال: 1999
ISSN: 1096-0929
DOI: 10.1093/toxsci/47.2.203